5,6-dihydroxyeicosatrienoic acid

5,6-dihydroxyeicosatrienoic acid (5,6-DHET) is an endogenous lipid mediator and a vicinal diol metabolite of arachidonic acid. It is produced via the cytochrome P450 (CYP) epoxygenase pathway, where arachidonic acid is first converted to 5,6-epoxyeicosatrienoic acid (5,6-EET) by CYP enzymes and subsequently hydrolyzed by soluble epoxide hydrolase (sEH) to form 5,6-DHET. While historically considered an inactive byproduct of the biologically active EETs, emerging research suggests that 5,6-DHET possesses distinct biological activities, particularly anti-inflammatory properties. In the context of inflammatory bowel disease research, 5,6-DHET has been shown to attenuate the production of neutrophil-attracting chemokines (such as CXCL-1) and modulate pro-inflammatory transcriptional networks involving TNFα, MAPK, and NF-κB in intestinal epithelial cells. It is primarily utilized as a research tool to study lipid signaling and metabolic inflammation.

Other names

5,6-DHET5,6-DiHET5,6-dihydroxy-8,11,14-eicosatrienoic acid(±)5,6-DHET