8-pMeOPT-2'-O-Me-cAMP

8-pMeOPT-2'-O-Me-cAMP (also known as 8-pMeOPT) is a cell-permeable, stable, and highly selective agonist of the Exchange Protein directly Activated by cAMP (Epac). It is a synthetic analog of cyclic AMP (cAMP) designed with a 2'-O-methyl modification that renders it nearly inactive toward Protein Kinase A (PKA), and an 8-para-methoxyphenylthio (pMeOPT) substitution that increases its affinity for Epac1 and Epac2. As a specialized research tool, it allows for the isolated study of Epac-mediated signaling pathways, such as those involving Rap1 activation, calcium mobilization, and cytoskeletal dynamics. In the context of oncology research, specifically melanoma, 8-pMeOPT has been used to demonstrate that Epac activation promotes cell migration through the PLCε/IP3 receptor 1 pathway and subsequent Ca2+-dependent actin assembly.

Other names

8-(4-Methoxyphenylthio)-2'-O-methyladenosine-3',5'-cyclic monophosphate8-(4-Methoxyphenylthio)-2'-O-methyl-cAMP8-pMeOPT-2'-OMe-cAMP