ATP-binding cassette transporter substrate-binding pocket (Clonostachys rosea) (ABC transporter SBP (C. rosea))

The ABC transporter substrate-binding pockets in the fungus Clonostachys rosea are critical structural domains within ATP-binding cassette (ABC) proteins that mediate the efflux of a wide range of toxic compounds (Nygren et al., 2018, Frontiers in Microbiology). Clonostachys rosea is a prominent mycoparasitic fungus used in biological control against plant pathogens, and its ability to survive in hostile environments is largely attributed to these transporters (Dubey et al., 2014, Molecular Plant-Microbe Interactions). The substrate-binding pockets, typically located within the transmembrane domains, recognize and bind xenobiotics such as fungicides (e.g., iprodione) and mycotoxins (e.g., zearalenone) produced by competing fungi (Karlsson et al., 2015, Genome Biology and Evolution). By facilitating the ATP-dependent removal of these substances from the cytoplasm, the binding pockets prevent cellular damage and ensure the fungus's efficacy as a biocontrol agent. Understanding the specificity and architecture of these pockets is essential for developing more resilient biocontrol strains and managing fungicide resistance in agricultural settings (Mamarot et al., 2021, Journal of Fungi). Research into these pockets often focuses on pleiotropic drug resistance (PDR) transporters, which are key players in the fungus's defensive and offensive strategies during mycoparasitism.

Other names

ABC transporter binding siteXenobiotic efflux pump binding pocketPDR-type ABC transporter substrate-binding domainGliocladium roseum ABC transporter pocket

The substrate-binding pocket facilitates the recognition and binding of xenobiotics or endogenous toxins, which are then actively transported across the plasma membrane using energy derived from ATP hydrolysis, thereby reducing intracellular concentration (Dubey et al., 2014, Molecular Plant-Microbe Interactions).