Bacterial ribosome subunit ()

Bacterial ribosome subunit Overview

The **bacterial ribosome** is a cytoplasmic nucleoprotein complex responsible for translating messenger RNA into proteins. It consists of two unequal-sized **subunits**—the **small 30S** and **large 50S**—which together form the functional **70S prokaryotic ribosome**. Each contains specific types of rRNAs and numerous proteins; for example, *E. coli*'s small unit has one molecule of 16S rRNA plus about twenty-one proteins, while its large unit contains one each of 23S and 5S rRNAs plus thirty-four proteins[1][2][6]. The main biological function is catalyzing peptide bond formation during translation—a process essential for cell survival—and decoding genetic information from mRNA templates. Both structural studies using cryo-electron microscopy and biochemical analyses have revealed detailed interactions with tRNAs, mRNAs, ions, antibiotics like paromomycin, as well as post-transcriptional modifications critical for function[2][3]. Because many antibiotics selectively bind these prokaryotic-specific structures without affecting eukaryotic cytoplasmic counterparts, they are major therapeutic targets against infectious diseases caused by bacteria. However, their similarity to mitochondrial counterparts poses safety challenges regarding off-target toxicity.[3]

Mechanism of Action

Drugs targeting bacterial ribosome subunits act primarily by inhibiting protein synthesis through mechanisms such as: – Blocking tRNA binding sites on the small or large rRNA components. – Disrupting peptide bond formation at the peptidyl transferase center. – Causing misreading of mRNA codons. – Preventing translocation along mRNA during translation.

Biological Functions

Safety Considerations

Interacting Drugs