Corneal epithelial basement membrane Overview
The corneal epithelial basement membrane is a specialized extracellular matrix between basal epithelial cells and the corneal stroma, composed principally of collagens, laminins, heparan sulfate proteoglycans (e.g., perlecan), and nidogens, with other components such as thrombospondin-1, matrilin-2/4, and sometimes fibronectin[1][3]. It anchors epithelium to stroma, supports epithelial migration and differentiation, and functions as a selective barrier and growth-factor reservoir that shapes homeostasis and wound healing[1][3]. Defective regeneration after injury allows epithelial- and tear-derived TGF-β and PDGF to persist in the stroma, driving myofibroblast development and corneal fibrosis/haze until the BM is fully restored and profibrotic signaling abates[3]. An intact BM also limits microbial translocation; disruption by injury or bacterial proteases facilitates penetration in infections such as Pseudomonas keratitis[4]. Epithelial reattachment involves reassembly of hemidesmosomes and anchoring fibrils; if the BM is damaged, firm adhesion can take months to re-establish[2].
Mechanism of Action
Restoration of BM integrity reduces stromal penetration of TGF-β/PDGF, depriving myofibroblasts of profibrotic cues and promoting apoptosis, which clears haze[3] Maintaining an intact BM provides a physical size-exclusion and matrix barrier that reduces microbial translocation across epithelium[4]
Biological Functions
Disease Associations
Safety Considerations
- Therapeutic challenge: severe epithelial/BM damage can take months or longer to re-establish firm epithelial-stromal adhesion, prolonging risk of erosions and haze[2][3]
- Disrupted BM permits excess stromal exposure to cytokines, sustaining fibrosis and delaying visual recovery[3]
- Infection risk increases when BM is breached; bacterial proteases (e.g., from Pseudomonas aeruginosa) can degrade BM components and enhance penetration[4]
Interacting Drugs
Associated Biomarkers
Biomarker |
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Presence/absence of key BM components (e.g., laminins, nidogen-1/2, perlecan) indicating BM regeneration status in healing cornea[3] |
Clinical haze/opacity and myofibroblast markers (vimentin, α-SMA, desmin) reflecting profibrotic signaling due to BM defects[3] |