Gosset Molecular Classification
Enzyme, Ligase, ATP-grasp superfamily, Acid-D-amino-acid ligase, Peptide synthase
Other Names
Alanine:alanine ligase (ADP-forming), Alanylalanine synthetase, D-Ala-D-Ala synthetase, D-alanylalanine synthetase, MurE synthetase, D-alanine:D-alanine ligase (ADP-forming)
Disease Roles
D-alanine--D-alanine Ligase Overview
D-alanine--D-alanine ligase (D-alanylalanine synthetase) is an essential bacterial enzyme that catalyzes the ATP-dependent formation of the dipeptide D-alanyl-D-alanine from two molecules of D-alanine. This reaction is a critical step in peptidoglycan biosynthesis, which is fundamental for bacterial cell wall integrity and survival. It is a validated target for antibiotics, such as D-cycloserine.
Mechanism of Action
Inhibition of D-alanyl-D-alanine synthesis by mimicking substrate or interfering with active site conformation.
Biological Functions
Peptidoglycan biosynthesis
Cell wall biogenesis
D-alanine metabolism
Disease Associations
Infection
Antibiotic resistance
Safety Considerations
- Development of resistance
- Restricted active site makes inhibitor design challenging
Interacting Drugs
D-cycloserine