Protein kinase, membrane-associated tyrosine- and threonine-specific 1 (PKMYT1)

Protein kinase, membrane-associated tyrosine- and threonine-specific 1 (PKMYT1) is a membrane-associated kinase of the Wee1 family that negatively regulates cell cycle progression by phosphorylating and inactivating cyclin-dependent kinase 1 (CDK1) at threonine 14 and tyrosine 15. Predominantly localized to the endoplasmic reticulum and Golgi apparatus, PKMYT1 functions in the cytoplasm and is essential for maintaining the G2/M checkpoint, preventing premature mitosis when DNA damage or errors are detected. It is frequently overexpressed in several cancer types, where it supports tumor cell proliferation, migration, and drug resistance, making it a promising therapeutic target for inhibitors in oncology research. PKMYT1 is regulated by polo-like kinase 1 (Plk1) and participates in a feedback loop with Wee1 and Cdc25 phosphatases to tightly control CDK1 activation and cell cycle transitions.

Other names

Myt1 kinasePKMYT1Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinaseprotein kinase Myt1membrane-associated tyrosine/threonine 1 kinase

Inhibition of CDK1 activity via phosphorylation at Thr14 and Tyr15, preventing premature mitosis. Checkpoint abrogation: Some drugs promote unscheduled CDK1 activation leading to cell death in cancer cells.