Tumor Antigen-Specific Chimeric Antigen Receptor (CAR) Signaling Domain (CAR Signaling Domain)

The signaling domain of a tumor antigen-specific chimeric antigen receptor (CAR) is a crucial component responsible for transducing signals upon antigen recognition. It typically comprises a combination of intracellular signaling domains, such as CD3ζ (for primary activation), CD28 or 4-1BB (for costimulation), and potentially OX40, to optimize T cell activation, proliferation, persistence, and anti-tumor efficacy. The specific combination of these domains can significantly impact the potency, durability, and safety profile of CAR-T cell therapies.

Other names

CAR intracellular domainCD28/OX40/CD3ζ CAR signaling domainChimeric Antigen Receptor signaling domain

Upon antigen binding, the CAR signaling domain initiates T cell activation through ITAM phosphorylation (CD3ζ), enhances proliferation and cytokine secretion (CD28), and promotes long-term survival/persistence (OX40).