16055 DG4 NFL clade C trimer

**16055 DG4 NFL clade C trimer** is a stabilized, cleavage-independent, native flexibly linked (NFL) HIV-1 envelope (Env) glycoprotein trimer derived from the clade C isolate 16055. It features structure-guided modifications including TD (trimer-derived) stabilizing mutations, CC (cysteine-cysteine) linkages for conformational stability, and a flexible glycine-serine linker replacing the furin cleavage site to enable proper folding, glycan processing, and quaternary assembly without co-transfection of furin protease. Produced at high yields (up to cGMP-compatible levels) in CHO cell lines, it displays a native-like closed structure, preferential recognition by broadly neutralizing antibodies (bNAbs) such as PGT145, PG16, PGDM1400, and VRC01, minimal binding by non-neutralizing antibodies (e.g., F105, V3-directed), and an oligomannose-dominated N-glycan shield (68% oligomannose-type). Developed as an HIV vaccine immunogen, it elicits robust tier 2 autologous neutralizing antibody responses against the 16055 virus in rabbits, non-human primates, and guinea pigs, with superior germinal center formation and neutralization when arrayed on liposomes or formulated in adjuvants like Matrix-M or SAS, while maintaining low V3-directed responses.

Other names

16055 NFL trimer