2C11xb12

2C11xb12 is a bispecific monoclonal antibody (bsAb) primarily utilized as a negative control in preclinical immunological research, specifically in syngeneic mouse models. It is engineered using the controlled Fab-arm exchange (cFAE) method to combine a mouse CD3ϵ-binding arm (derived from the Armenian hamster monoclonal antibody clone 145-2C11) with a non-mammalian target-binding arm (derived from the human HIV-1 gp120-specific monoclonal antibody clone b12). The antibody is constructed on a mouse IgG2a backbone containing L234A and L235A (LALA) mutations to silence Fc-mediated effector functions, such as antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC). In mouse tumor studies, 2C11xb12 serves to isolate the effects of T-cell redirection to a tumor-associated antigen (TAA) from the effects of systemic T-cell activation or residual Fc-receptor interaction, as its second target (gp120) is absent in the mouse environment.

Other names

anti-CD3 x anti-gp120 bispecific antibodyanti-CD-3 x anti-gp120 bispecific antibodyanti-CD 3 x anti-gp120 bispecific antibodyCD3xb12CD-3xb12CD 3xb12