AAV8-eGFP

AAV8-eGFP is a recombinant gene therapy vector based on adeno-associated virus serotype 8 (AAV8) that carries and expresses the gene encoding enhanced green fluorescent protein (eGFP) as a reporter. The vector delivers the eGFP gene under the control of various promoters (e.g., ubiquitous CMV or CAG, or cell-specific promoters) to allow for the visualization and quantification of gene transfer and expression in mammalian tissues, typically in preclinical and research contexts. AAV8 exhibits high transduction efficiency, especially in neurons and astrocytes in the central nervous system and hepatocytes in the liver. The mechanism of action is based on cell entry by AAV8 capsids, leading to introduction and transient expression of the eGFP protein in mammalian cells. This construct is widely used for in vivo model studies, promoter specificity assays, and as a control in gene therapy research, but is not intended as a therapeutic on its own.[1][2][5]

Other names

adeno-associated virus serotype 8 expressing enhanced green fluorescent proteinAAV8-GFPAAV-8-GFPAAV 8-GFPAAV8 CMV-eGFPAAV-8 CMV-eGFPAAV 8 CMV-eGFPAAV8-GFP reporter vectorAAV-8-GFP reporter vectorAAV 8-GFP reporter vector