Ad-siNT

Ad-siNT is a recombinant adenoviral vector, typically based on the Adenovirus Serotype 5 (Ad5) genome, designed to deliver and express a non-targeting (NT) small interfering RNA (siRNA) or short hairpin RNA (shRNA) sequence. It serves as a standard negative control in preclinical research involving gene silencing and viral-mediated gene therapy. By delivering an RNA sequence that has no known complementary targets in the mammalian transcriptome, Ad-siNT allows researchers to control for the biological effects associated with adenoviral transduction, such as the induction of innate immune responses, and the general activation of the RNA-induced silencing complex (RISC). In studies of tumor biology and chemoresistance, such as those focusing on the NF-kappaB pathway in non-small cell lung cancer (NSCLC) or the use of adenoviral aptamers like A-p50, Ad-siNT is frequently used to establish a baseline for comparing the therapeutic efficacy of gene-specific vectors. While primarily a research tool, it is essential for the validation of viral-delivered RNA therapeutics.

Other names

Ad-si-NTAdenovirus-non-targeting siRNAAd-control siRNA