autologous dedifferentiated fat cells

Autologous dedifferentiated fat cells (DFAT cells) are a unique population of multipotent progenitor cells derived from mature adipocytes through a process called ceiling culture. Unlike traditional adipose-derived stem cells (ASCs) which are isolated from the stromal vascular fraction, DFAT cells are produced by inducing mature, lipid-filled fat cells to undergo dedifferentiation, reverting them to a fibroblast-like multipotent state. These cells exhibit high purity and a potent capacity for angiogenesis and tissue regeneration. In the treatment of peripheral artery disease (PAD) and critical limb ischemia (CLI), DFAT cells are typically administered via intramuscular injection into the affected limb. Their therapeutic effect is primarily mediated through paracrine signaling, involving the secretion of high levels of angiogenic factors such as vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF), as well as their ability to differentiate into vascular endothelial cells and smooth muscle cells to facilitate revascularization.

Other names

dedifferentiated adipocytesDFAT cellsceiling culture-derived cells