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Biphasic human insulin 30 is a pre-mixed (biphasic) formulation containing two types of recombinant human insulins in fixed proportions—typically **30% regular (short-acting) human insulin and 70% isophane (NPH; intermediate-acting) human insulin**. This combination provides both rapid onset and prolonged duration of action to help control blood glucose levels in people with diabetes mellitus. The short-acting component covers post-meal glucose spikes while the intermediate-acting component maintains basal glycemic control between meals and overnight. Biphasic insulins are administered subcutaneously—usually twice daily before meals—and are used primarily for the management of type 1 and type 2 diabetes mellitus[1][3][5][9]. The mechanism of action involves mimicking endogenous pancreatic insulin by promoting cellular uptake of glucose from the bloodstream into tissues such as muscle and fat while inhibiting hepatic glucose production.
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