Cas9 ribonucleoprotein

Cas9 ribonucleoprotein (RNP) is a genome-editing complex consisting of the Cas9 nuclease protein physically complexed with a guide RNA (gRNA). Unlike plasmid or viral delivery of CRISPR components, RNP delivery allows for immediate, transient gene editing, which reduces the risk of off-target effects and insertional mutagenesis. In the context of research from Clemson University, Cas9 RNPs are being packaged into specialized peptide nanocarriers, such as the fusogenic peptide DIV3W and the brain-targeting RVG29-DIV3W, to treat glioblastoma. These carriers are designed to bypass the blood-brain barrier and target NCAM1-expressing tumor cells to deliver RNPs that can knockout therapeutic targets like PLK1.

Other names

Cas9 RNPCas-9 RNPCas 9 RNP