CD19-CAR lentiviral vector

CD19-CAR lentiviral vector is a gene therapy tool used to genetically engineer T cells to express a chimeric antigen receptor (CAR) directed against the human CD19 antigen. The vector is typically a self-inactivating (SIN) lentivirus, often pseudotyped with the vesicular stomatitis virus G glycoprotein (VSV-G), which delivers a transgene cassette encoding an anti-CD19 single-chain variable fragment (scFv) linked to intracellular signaling domains such as 4-1BB (CD137) or CD28 and the CD3ζ chain. Once transduced, the patient's T cells (CAR-T cells) can specifically recognize and eliminate CD19-expressing B cells. This platform is widely utilized in the development of therapies for B-cell malignancies, including acute lymphoblastic leukemia (ALL) and B-cell lymphomas, as well as for refractory systemic autoimmune diseases where B-cell depletion is therapeutic.