Chloroform

Chloroform (CHCl3) is a chemical compound widely used as a solvent in various scientific and industrial applications. In the provided contexts, it is frequently employed for the extraction of bioactive compounds from diverse plant sources, such as *Tripterygium wilfordii*, *Annona muricata*, *Leuenbergeria bleo*, *Ruellia simplex*, *Equisetum hyemale L.*, *Stachys rupestris*, *Inula species*, and *Asphodelus tenuifolius Cav.*, which are subsequently investigated for their potential therapeutic properties. It also serves as a solvent in the preparation of advanced materials like polycaprolactone-bioceramic composites and metal complexes, and is utilized in molecular biology for DNA isolation through phenol-chloroform extraction. Furthermore, Chloroform is recognized as an ozone-depleting substance and an emerging pollutant, with ongoing monitoring of its emissions.