CK-666

CK-666 is a cell-permeable, selective small molecule inhibitor of the actin-related protein 2/3 (Arp2/3) complex. It was discovered through high-throughput screening of a small molecule library by Cytokinetics in collaboration with Yale University. CK-666 binds to a pocket at the interface between the Arp2 and Arp3 subunits, stabilizing the complex in its inactive conformation and preventing the structural transition into the active, short-pitch conformation required for branched actin filament nucleation. It is widely used as a chemical probe in cell biology research to study actin cytoskeleton dynamics, cell migration, endocytosis, and intracellular pathogen motility (such as *Listeria monocytogenes*).

Other names

2-fluoro-N-(2-(2-methyl-1H-indol-3-yl)ethyl)benzamide2-fluoro-N-[2-(2-methyl-1H-indol-3-yl)ethyl]-benzamideActin Assembly Inhibitor XIII