dCas9-HDAC2

dCas9-HDAC2 is an epigenome editing tool consisting of a catalytically inactive Cas9 (dCas9) protein fused to the histone deacetylase 2 (HDAC2) enzyme. This chimeric protein is directed to specific genomic sequences by a guide RNA (gRNA). Once bound to the target promoter or enhancer, the HDAC2 domain removes acetyl groups from lysine residues on histone tails (such as H3K27ac), leading to chromatin condensation and transcriptional repression of the target gene. This approach, often categorized under CRISPR interference (CRISPRi), allows for precise, sequence-specific gene silencing without altering the underlying DNA sequence. It is primarily used in preclinical research to study gene function and is being explored for potential therapeutic applications in diseases driven by gene overexpression, such as certain cancers and neurological disorders.

Other names

CRISPRi-HDAC2CRISPRi-HDAC-2CRISPRi-HDAC 2dCas9-fused HDAC2dCas-9-fused HDAC2dCas 9-fused HDAC2dead Cas9-HDAC2