E20-SNP

E20-SNP is a charge-switching lipid nanoparticle (SNP) designed for the efficient delivery of nucleic acids, such as mRNA and nanoplasmid DNA, with significantly reduced immunogenicity compared to traditional cationic lipid nanoparticles (LNPs). Developed by researchers at UC Berkeley and collaborating institutions, E20-SNP utilizes a novel ionizable lipid (S-lipid) featuring a carboxylic acid head-group. This lipid is negatively charged at physiological pH (7.4), which prevents the activation of inflammatory pathways frequently triggered by traditional LNPs, such as TLR4, complement, and galectin-8. Upon internalization into endosomes, the lipid switches to a positive charge at pH 4.0, facilitating endosomal escape and the release of its genetic cargo. In preclinical models, E20-SNPs carrying IL-22 mRNA have demonstrated the ability to treat LPS-induced acute lung injury without exacerbating pre-existing inflammation, while those carrying nanoplasmid DNA have shown efficient liver transfection with reduced toxicity.

Other names

E20-SNP lipid nanoparticleE-20-SNP lipid nanoparticleE 20-SNP lipid nanoparticleE20-SNP LNPE-20-SNP LNPE 20-SNP LNP