FastAd

FastAd is a rapid platform for adenoviral capsid engineering and fiber modification developed at the Mayo Clinic. The technology utilizes a recombinase-mediated system to knock in donor DNA directly into an infectious "receiver" adenovirus within mammalian cells. This approach significantly accelerates the production of modified adenoviral vectors, reducing the timeline from approximately two months to just 10 days. FastAd is designed to circumvent the limitations of conventional rescue workflows, particularly when major capsid modifications reduce viral fitness. It has been applied to generate complex libraries, including barcodes, gRNAs, cDNAs, and retargeted fiber proteins, facilitating the development of vectors with altered tropism for gene therapy applications.