HDAd.Cre

HDAd.Cre is a helper-dependent adenoviral (HDAd) vector designed to express Cre recombinase. HDAd vectors, also known as "gutless" adenoviruses, have all viral coding sequences removed, which significantly reduces immunogenicity and provides a large packaging capacity (up to 36 kb). In the context of the provided research, HDAd.Cre is utilized as a control or a validation tool for the in vivo engineering of hematopoietic stem and progenitor cells (HSPCs). It is used to demonstrate gene delivery and site-specific recombination in reporter mouse models (such as hCD46/Ai9), where Cre activity triggers the expression of fluorescent proteins like tdTomato. This vector is part of a broader platform developed by researchers at the University of Washington to enable the sustained production of engineered extracellular vesicles for the treatment of lysosomal storage disorders, including Pompe Disease.

Other names

Helper-dependent adenoviral vector expressing Cre recombinase