hemp seed protein hydrolysate fraction F3

Hemp seed protein hydrolysate fraction F3 is a bioactive peptide-rich extract derived from the enzymatic hydrolysis of hemp seed (*Cannabis sativa* L.) protein isolate. It is typically produced through a sequential digestion process using pepsin and pancreatin to simulate human gastrointestinal digestion, achieving a degree of hydrolysis of approximately 48%. The resulting hydrolysate is then fractionated using ultrafiltration or chromatography. In a three-fraction separation system, F3 represents the high-molecular-weight component (typically >10 kDa). Preclinical research has identified this specific fraction as possessing the most potent antiproliferative activity among hemp protein derivatives, demonstrating significant growth inhibition in cancer cell lines such as Caco-2 (colorectal cancer) and THP-1 (leukemia). While lower molecular weight fractions exhibit higher antioxidant capacity, F3 is primarily characterized by its potential chemoprotective and antineoplastic properties, making it a subject of interest for functional food and nutraceutical applications in oncology.

Other names

hemp seed peptide fraction F3high-molecular-weight hemp protein hydrolysate fractionhemp seed protein hydrolysate fraction >10 kDa