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HiLyte Fluor 750 is a near-infrared (NIR) fluorescent dye with excitation and emission wavelengths typically around 750 nm and 780 nm, respectively. It is designed for high-sensitivity imaging applications, including in vivo imaging, due to its low background interference in the NIR spectrum. In research, it is often conjugated to peptides, antibodies, or other targeting ligands to create molecular probes for detecting specific biomarkers, such as the urokinase plasminogen activator (uPA) receptor in pancreatic adenocarcinoma. It is commonly used in techniques like Multispectral Tomography (MSOT) and fluorescence microscopy.
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