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Humulus oil is the essential oil extracted from the strobiles (cones) of the *Humulus lupulus* plant, commonly known as hops. It is a complex mixture of volatile compounds, primarily sesquiterpenes and monoterpenes such as alpha-humulene, beta-caryophyllene, and myrcene. Historically used in the brewing industry for aroma and preservation, humulus oil is also utilized in herbal medicine for its sedative, anxiolytic, and antimicrobial properties. Its mechanism of action involves the modulation of GABAergic signaling, contributing to its sleep-inducing effects, and it contains prenylated flavonoids like xanthohumol and 8-prenylnaringenin which exhibit antioxidant and potent phytoestrogenic activities. The oil is frequently found in over-the-counter dietary supplements and aromatherapy products aimed at improving sleep quality and managing menopausal symptoms.
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