LaG16Notch

LaG16Notch is a synthetic Notch (synNotch) receptor construct utilized as a negative control in the development of engineered microglial therapies for Alzheimer's disease. Developed by researchers at Vanderbilt University, the receptor consists of an extracellular sensing domain derived from the LaG16 nanobody, which specifically binds to Green Fluorescent Protein (GFP), fused to a Notch receptor scaffold and an intracellular transcriptional domain. In studies evaluating amyloid-beta (Aβ) responsive microglia, LaG16Notch serves to demonstrate the specificity of Aβ-targeting receptors (such as BapNotch) by showing no activation or downstream transgene expression (e.g., mCherry or luciferase) upon exposure to Aβ42. This construct is a critical tool for validating the precision of context-specific neuroimmune engineering platforms designed to modulate neuroinflammation in neurodegenerative disorders.