lipid nanoparticle-encapsulated cre recombinase mRNA

Lipid nanoparticle-encapsulated Cre recombinase mRNA is a specialized research tool and technology platform used primarily in preclinical studies to evaluate the performance of mRNA delivery systems. It consists of messenger RNA (mRNA) encoding the Cre recombinase enzyme, which is protected and delivered by a lipid nanoparticle (LNP) shell. Once the LNPs are internalized by cells, the mRNA is released and translated into the Cre recombinase protein. This protein then catalyzes site-specific DNA recombination at loxP sites, typically resulting in the expression of a reporter gene (such as luciferase or a fluorescent protein) in genetically engineered "Cre-lox" reporter mice (e.g., Ai9 or Ai14 strains). This technology is essential for optimizing LNP formulations, assessing organ-specific targeting, and confirming functional protein expression in vivo. While not a therapeutic candidate itself, it is a standard benchmark in the development of mRNA-based therapies for conditions like sickle cell disease.

Brand names

HiExpress Cre LNP-mRNA

Other names

Cre mRNA LNPsCre-mRNA containing LNPsCre recombinase mRNA LNP