N-hydroxydansylamide

N-hydroxydansylamide (DAN-HNO) is a profluorescent azanone (nitroxyl, HNO) donor developed for the real-time visualization of HNO release and the study of HNO biology. Under physiological conditions (37°C, pH 7.4), N-hydroxydansylamide decomposes with a half-life of approximately 20 to 33 minutes to release a molecule of HNO and a fluorescent dansyl-based sulfinate derivative, allowing fluorometric monitoring of HNO release without depleting the HNO source. In vitro studies have demonstrated that HNO released from N-hydroxydansylamide exhibits potent antithrombotic activity by concentration-dependently inhibiting coagulation and activating fibrinolysis, potentially through the inhibition of platelet activity and fibrin polymerization.