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Nitrate-depleted beetroot juice is a research-grade placebo used in clinical trials to evaluate the physiological effects of dietary inorganic nitrate. It is produced by removing the majority of inorganic nitrate (NO3−) from beetroot juice, typically reducing levels from approximately 300–400 mg to about 40 mg per 70 mL serving. This allows researchers to maintain the sensory properties (taste, color, and smell) of the juice while eliminating the active precursor to the nitric oxide (NO) signaling pathway. It has been used extensively in studies at The Pennsylvania State University to investigate cardiovascular health, specifically focusing on endothelial function and blood pressure in postmenopausal women.
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