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**Protein yeast hydrolysate** is a mixture of peptides, amino acids, and other nutrients obtained by enzymatic or chemical hydrolysis of yeast protein, commonly sourced from *Saccharomyces cerevisiae*. The process employs endopeptidases and exopeptidases (such as alcalase, neutrase, flavourzyme, bromelain) to break down yeast proteins into smaller, more bioavailable peptides and amino acids[1][4]. Protein yeast hydrolysate serves as a functional food ingredient, dietary supplement, and cell culture additive. It exhibits nutritional benefits due to high essential amino acid content and is used as a protein source in livestock feed, functional or medical foods, and as an additive in mammalian cell cultures for biologics and vaccine production[1][2]. It is also used for weight management, anti-obesity supplementation, improving gut and immune health, and as a flavor enhancer due to its umami properties[3][4][5][6]. Mechanistically, its bioactivity is attributed to the high content of bioavailable amino acids, small peptides, nucleotides, and bioactive compounds that can impact satiety (for obesity), support cellular and immune functions, and promote cell growth in vitro[2][4][6].
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