RPC-bPEI 0.8 kDa

RPC-bPEI 0.8 kDa is a research-stage reducible polycationic gene delivery vector synthesized from low molecular weight (0.8 kDa) branched polyethyleneimine (bPEI) through thiolation and oxidation. This process creates a higher molecular weight polymer (5-80 kDa) containing disulfide bonds that are stable in the extracellular environment but undergo reduction and cleavage within the intracellular space. This 'reducible' property allows for the efficient release of genetic cargo, such as plasmid DNA or nanostructured oligonucleotides, into the cytoplasm while significantly minimizing the cytotoxicity typically associated with non-degradable high molecular weight PEI. The vector effectively condenses nucleic acids into nanoparticles (100-200 nm) that exhibit high transfection efficiency. In preclinical studies, it has been used to deliver Y-shaped DNA (forming the YbNano complex) to activate innate immune pathways, specifically cGAS/STING and TLR9, for the treatment of melanoma and metastatic breast cancer.

Other names

reducible polycationic gene vectorreducible polycationic branched polyethyleneimine 0.8 kDa