shRNA V0a2

shRNA V0a2 is an experimental short hairpin RNA (shRNA) construct designed to silence the expression of the a2 subunit of the vacuolar H+-ATPase (V-ATPase), which is encoded by the *ATP6V0A2* gene. The V-ATPase is a multi-subunit proton pump responsible for the acidification of intracellular compartments, including lysosomes and the Golgi apparatus. The a2 isoform (V0a2) is specifically localized to the Golgi and is frequently overexpressed in various malignancies, such as breast, ovarian, and prostate cancers. In these contexts, V0a2 contributes to the abnormal glycosylation of surface proteins and promotes an invasive phenotype by modulating the tumor microenvironment and intracellular trafficking. By utilizing RNA interference (RNAi), shRNA V0a2 achieves stable knockdown of the target protein, typically through delivery via lentiviral or adenoviral vectors. This tool is primarily used in preclinical research to investigate the role of pH regulation in oncogenesis and to evaluate the therapeutic potential of targeting the V-ATPase machinery to inhibit tumor cell invasion and growth.

Other names

ATP6V0A2 shRNAATP-6V0A2 shRNAATP 6V0A2 shRNAV-ATPase a2 shRNAV0a2 shRNAV-0a2 shRNAV 0a2 shRNAshRNA-mediated knockdown of V0a2