TIM4 BiTE

TIM4 BiTE is an experimental bispecific T-cell engager (BiTE) designed to target phosphatidylserine (PS) on the surface of tumor cells. PS is typically restricted to the inner leaflet of the plasma membrane but becomes exposed on the surface of tumor cells due to cellular stress, radiation, or chemotherapy. The construct consists of the extracellular domain of the natural high-affinity PS receptor, T-cell immunoglobulin and mucin domain-containing protein 4 (TIM4), linked to a single-chain variable fragment (scFv) targeting CD3 on T cells. By binding both PS and CD3, the TIM4 BiTE redirects T-cell cytotoxicity toward tumor cells, bypassing the need for specific neoantigens and potentially overcoming intratumoral heterogeneity. It has shown efficacy in preclinical models of glioblastoma.