5-oxoprolinase (OPLAH)

5-oxoprolinase is a cytoplasmic ATP-dependent enzyme essential for converting 5-oxo-L-proline to L-glutamate, operating as part of the gamma-glutamyl cycle that maintains glutathione homeostasis and amino acid metabolism[4][5][6][8]. Deficiency results in excess 5-oxoproline (5-oxoprolinuria), a rare inherited metabolic disorder with diverse systemic and neurological symptoms, highlighting the enzyme's importance in cellular redox balance and metabolic stress response[8].

Other names

5-oxo-L-prolinase5-oxo-L-proline amidohydrolase (ATP-hydrolyzing)5-OPaseOPLAHpyroglutamasepyroglutamate hydrolaseL-pyroglutamate hydrolase

For inhibitors, competitive inhibition of the enzyme’s ATP-dependent hydrolytic activity, resulting in accumulation of 5-oxoproline and interfering with glutathione recycling