Advanced platelet-rich fibrin (A-PRF)

Advanced platelet-rich fibrin (A-PRF) is an autologous, second-generation platelet concentrate obtained from the centrifugation of peripheral blood without the use of anticoagulants or bovine thrombin (Ghanaati et al., 2014). It functions as a three-dimensional fibrin scaffold that entraps platelets, leukocytes, and a variety of growth factors, including transforming growth factor-beta (TGF-β), platelet-derived growth factor (PDGF), and vascular endothelial growth factor (VEGF) (Miron et al., 2017). The advanced protocol utilizes lower centrifugation speeds compared to traditional PRF, which results in a more porous fibrin network and a higher entrapment of leukocytes, which are critical for the early stages of wound healing and immune regulation. A-PRF is not a molecular target but rather a therapeutic biomaterial used in regenerative medicine to promote angiogenesis and tissue repair. Its scaffold-level interactions involve providing a physical matrix for cell migration and a biochemical reservoir for the slow release of regenerative signals (Choukroun & Ghanaati, 2018). This material is widely utilized in oral and maxillofacial surgery, orthopedics, and wound management to accelerate tissue repair and improve surgical outcomes. Because it is derived from the patient's own blood, it minimizes the risk of immune rejection or disease transmission. The efficacy of A-PRF is highly dependent on the centrifugation parameters and the immediate handling of the blood sample.

Other names

A-PRFAdvanced PRFPlatelet-rich fibrin scaffoldChoukroun's PRF

Advanced platelet-rich fibrin acts as a bioactive three-dimensional scaffold that provides sustained release of growth factors and cytokines while supporting cell adhesion, migration, and differentiation.