B-cell receptor specific for prefusion human metapneumovirus fusion protein (BCR-hMPV-preF)

The B-cell receptor (BCR) specific for the prefusion human metapneumovirus (hMPV) fusion (F) protein is a membrane-bound immunoglobulin that recognizes specific antigenic determinants on the viral surface [1]. The hMPV F protein is a class I fusion protein that mediates viral-cell membrane fusion, and its prefusion conformation is the primary target for potent neutralizing antibodies [2]. Recognition of prefusion-specific epitopes, such as Site Ø or Site V, by these BCRs is essential for the induction of a protective immune response [3]. Therapeutic strategies, particularly vaccine development, aim to engage these specific BCRs using stabilized prefusion F immunogens to elicit high titers of neutralizing antibodies [4]. This approach is critical for addressing hMPV-related respiratory tract infections, which pose a significant burden on pediatric and elderly populations [5]. Upon binding the prefusion F antigen, the BCR initiates signaling pathways that lead to B-cell expansion and maturation into plasma cells. These plasma cells then secrete soluble antibodies that neutralize the virus by preventing the conformational change required for membrane fusion. Understanding the interaction between the BCR and the prefusion F protein is vital for designing next-generation vaccines that provide broad and durable protection against hMPV.

Other names

hMPV F-specific B-cell receptorPrefusion hMPV F-specific BCRHuman metapneumovirus fusion protein-specific B-cell receptorhMPV preF-specific BCR

The mechanism involves the binding of the prefusion hMPV F protein (the immunogen) to the B-cell receptor, which triggers receptor clustering and activates intracellular signaling cascades (e.g., via Lyn, Syk, and PLCγ2), leading to B-cell proliferation, affinity maturation, and differentiation into plasma cells that secrete neutralizing antibodies [1, 2].