B16-F10 melanoma cell line (B16-F10)

B16-F10 is a syngeneic murine melanoma cell line derived from the C57BL/6 mouse strain, widely recognized in preclinical oncology for its high metastatic potential and aggressive growth characteristics [ATCC CRL-6475]. It was originally established through serial transplantation and selection for lung-colonizing ability, making it a standard model for studying the cellular and molecular mechanisms of cancer metastasis [Fidler, 1973]. Because it can be grown in immunocompetent C57BL/6 mice, it serves as a primary tool for evaluating cancer immunotherapies, including checkpoint inhibitors and therapeutic vaccines [Overwijk & Restifo, 2001]. The cells are characterized by their production of melanin and the expression of melanoma-specific antigens such as tyrosinase-related protein 1 (TRP-1) and gp100. It is important to note that B16-F10 is a biological model system (cell line) rather than a single molecular target like a receptor or enzyme.

Other names

B16F10B16 mouse melanomaB16-F10 murine melanomaB16-F10 cell line

B16-F10 is not a single molecular target; however, it is used as a model system to evaluate drugs that target PD-1/PD-L1 signaling, CTLA-4, or other immune-oncology pathways to restore anti-tumor immune responses [Overwijk & Restifo, 2001].