Beta-mannanase (Aspergillus oryzae) (AoMan5A)

Beta-mannanase from Aspergillus oryzae (specifically the AoMan5A isoform) is a glycoside hydrolase belonging to family 5 (GH5) that specializes in the endohydrolysis of beta-1,4-D-mannosidic linkages within various mannan-based polysaccharides (UniProt Q2U064). This enzyme is essential for the degradation of plant cell wall components, making it highly valuable in industrial biotechnology for processes such as the bleaching of paper pulp, the improvement of animal feed digestibility, and the production of prebiotic mannooligosaccharides (PMID: 22900545). The interaction between the enzyme and its substrate mannotriose—a trisaccharide of beta-1,4-linked mannose—serves as a critical model for understanding the enzyme's active site architecture and its catalytic mechanism, which involves a double-displacement reaction that retains the anomeric configuration (PDB 3WH9). While not a traditional therapeutic target for human disease, it is occasionally explored as a digestive enzyme supplement to assist in the breakdown of complex dietary carbohydrates (PubChem CID 439336). Safety considerations are primarily focused on its potential as a respiratory allergen in industrial environments where fungal proteins are aerosolized (PMID: 11422141). The input name refers specifically to a structural complex of the enzyme with its substrate rather than a canonical target entity.

Other names

AoMan5AEndo-1,4-beta-mannanaseMannan endo-1,4-beta-mannosidase1,4-beta-D-mannan mannanohydrolase

Not applicable as there are no known therapeutic drugs targeting this fungal enzyme.