Cellular RNAs other than IDO1 mRNA

Cellular RNAs other than IDO1 mRNA is a collective term used in pharmacology and molecular biology to describe the entire pool of RNA molecules within a cell, excluding the specific messenger RNA that encodes the Indoleamine 2,3-dioxygenase 1 (IDO1) enzyme. This category is primarily relevant in the development of RNA-targeted therapeutics, such as antisense oligonucleotides (ASOs), small interfering RNAs (siRNAs), or RNA-binding small molecules, where it serves as the off-target population (Source: Nucleic Acids Research, 2020). IDO1 is a key metabolic enzyme involved in the kynurenine pathway, often overexpressed in the tumor microenvironment to facilitate immune escape by depleting tryptophan and producing immunosuppressive metabolites (Source: Nature Reviews Cancer, 2017). While therapeutic strategies aim to selectively degrade or inhibit the translation of IDO1 mRNA to restore anti-tumor immunity, avoiding interactions with other cellular RNAs is essential to prevent systemic toxicity and maintain the safety profile of the drug. Consequently, this group of RNAs is used in specificity assays to ensure that the drug candidate does not cause unintended gene knockdown or trigger innate immune responses through non-specific binding (Source: PubMed, PMC4915101).

Other names

Non-target RNAsOff-target transcriptomeCellular RNAs excluding IDO1Non-specific cellular RNA

Not applicable as this represents a non-target group used for specificity assessment.