CRISPR–Cas target sequence (null)

The **CRISPR–Cas target sequence** is a short, user-selected segment of DNA (or, in specific Cas systems, RNA) in a cell’s genome that is recognized through base pairing with the guide RNA of a CRISPR–Cas system and subsequently cleaved or edited by the associated Cas protein[2][6][7][8]. The target sequence must be adjacent to a specific short motif (the PAM sequence for most Cas systems), and its precise genetic context is selected according to experimental or therapeutic objectives. The sequence itself is not a biological entity, but rather a designation for any site that is intentionally targeted for genome engineering. Safety and efficacy of targeting depend heavily on the specificity of guide RNA design and the molecular context of cleavage.

Other names

target DNA sequenceprotospacer sequencetarget locustarget RNA sequence

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