DNA-directed RNA polymerase II subunit G (POLR2G)

DNA-directed RNA polymerase II subunit G (POLR2G) encodes the seventh largest subunit of RNA polymerase II, the complex required for the synthesis of messenger RNA in eukaryotes. POLR2G (also referred to as RPB7) is a core enzymatic component that participates in both transcription initiation and the stabilization of the elongating polymerase complex during RNA synthesis. Structural studies show RPB7 binds RNA as it emerges from the polymerase and interacts with the RPB4 subunit to maintain the enzyme's closed conformation and facilitate RNA binding. POLR2G is essential for cell viability, and its depletion results in cytotoxicity and gene-specific transcriptional defects. Beyond its canonical role in transcription, altered POLR2G expression and methylation is associated with metabolic disorders, notably gestational diabetes mellitus. The subunit interacts functionally within the polymerase II complex but does not have known direct drug interactors or clinical biomarkers beyond gene expression/methylation monitoring. Safety concerns primarily arise from the global toxicity associated with inhibition of the transcriptional machinery.

Other names

DNA-directed RNA polymerase II subunit RPB7RPB7RNA polymerase II subunit B7RPB19hRPB19hsRPB7RNA polymerase II 19 kDa subunitRNA polymerase II seventh subunitpolymerase (RNA) II (DNA-directed) polypeptide Gpolymerase (RNA) II subunit G

General mechanism includes inhibition of RNA polymerase II activity, not specific to POLR2G subunit; α-amanitin binds RNA polymerase II and blocks transcription