DNA double-strand at user-specified genomic locus (null)

This entity represents a generic double-stranded DNA segment at any specified locus within a genome that is subject to site-specific cleavage using a programmable system such as CRISPR-Cas9 or CRISPR-Cas12a. In these genome editing technologies, a guide RNA (gRNA) directs an endonuclease (often Cas9 or Cas12a) to the genomic sequence of interest, where the enzyme generates a double-strand break (DSB)[1][3][5][6][9]. The DNA itself is not a therapeutic target; rather, the true target is the programmable endonuclease (e.g., Cas9, Cas12a), which acts upon the DNA specified by the guide RNA. The description provided is non-standard as a "target" entry, since it does not refer to a particular biological macromolecule or protein, but to a sequence-selective site defined by user input. Therefore, it is best treated as a non-canonical, incorrect entry for the context of therapeutic targeting. Key supporting references: - CRISPR-Cas9 and related systems cleave double-stranded DNA at user-directed sites through gRNA-directed interaction of the endonuclease with a specific genomic sequence[1][3][5][9]. - The DNA locus is not a protein, receptor, enzyme, or other classical drug target, but rather a site specified in a genome editing experiment[1][3][5][9].

Other names

genome DNA double-strandtargeted DNA locusCRISPR target site