Molecular Classification
Enzyme, DNA Polymerase
Other Names
Pol II, polB gene product
Disease Roles
Response to DNA damageMutagenesis (indirectly)

DNA Polymerase II Overview

DNA polymerase II (Pol II) is a prokaryotic DNA-dependent DNA polymerase primarily involved in DNA repair and acts as a backup enzyme for the main replicative polymerase, DNA polymerase III, in *Escherichia coli*. It catalyzes phosphodiester bond formation between nucleotides during both normal replication (as backup) and repair synthesis. It possesses 5'→3' polymerase activity and 3'→5' exonuclease (proofreading) activity. Its processivity is enhanced by interaction with accessory proteins such as the β-clamp and clamp loader complex.

Mechanism of Action

Inhibition of DNA synthesis through competitive or non-competitive mechanisms

Biological Functions

DNA replication (backup)
DNA repair
Proofreading

Disease Associations

Response to DNA damage
Mutagenesis (indirectly)

Safety Considerations

  • Potential for off-target effects on other DNA polymerases
  • Induction of mutations if repair function is compromised