Enoyl-acyl-carrier protein reductase (FabI)

Enoyl-acyl carrier protein reductase (FabI) is a key enzyme in the type II fatty acid synthesis (FAS II) pathway. It catalyzes the final reduction step in each cycle of fatty acid elongation, converting trans-2-enoyl-ACP substrates to fully saturated acyl-ACP products. This reaction is essential for bacterial viability and growth, making FabI an attractive target for antibacterial drug development due to its sequence conservation across many pathogenic bacterial species. FabI utilizes NADH or NADPH as electron donors. Inhibitors targeting this enzyme include diazaborines and AFN-1252; resistance mutations often map directly onto fabI.

Other names

ENREnoyl-ACP reductase

Inhibition of enoyl-acyl-carrier protein reductase, blocking fatty acid synthesis.