Fluorescent imaging

Fluorescent imaging is a non-invasive technique used to visualize and quantify cellular and molecular processes in biological samples or living organisms. It operates by exciting fluorophores—molecules that can re-emit light when illuminated at specific wavelengths—allowing for highly specific and dynamic observation of biological structures and events in real time. Applications span cell and molecular biology, disease diagnosis, drug tracking, and in vivo monitoring of physiological or pathological processes. Fluorescent imaging techniques include fluorescence microscopy, in vivo fluorescence imaging, and specialized assays using fluorescent proteins or dyes. These methods are central to research in oncology, neuroscience, immunology, and many other fields, enabling studies of cell signaling, gene expression, protein localization, molecular interactions, and the biodistribution of therapeutics. Common methods include immunofluorescence (antibody-based), fluorescence in situ hybridization (FISH), and live-cell imaging with fluorescent proteins such as GFP[1][2][3][4][5][6][7][8].

Other names

Fluorescence microscopyFluorescence imagingFluorescence-based imagingFluorescent labelingIn vivo fluorescence imaging