Host translation machinery producing enhanced GFP

The host translation machinery refers to the complex assembly of ribosomes, transfer RNAs (tRNAs), and various translation factors (initiation, elongation, and termination) that synthesize proteins from messenger RNA (mRNA) templates [1]. In this specific context, the machinery is utilized to express Enhanced Green Fluorescent Protein (eGFP), a common reporter used in high-throughput screening to monitor the efficiency of protein synthesis or the impact of potential inhibitors [2]. While the ribosome itself is a well-known target for antibiotics, the host (eukaryotic) translation machinery is often targeted in the context of viral infections, as viruses are obligate parasites that hijack this system to produce viral proteins [3]. Additionally, dysregulation of translation factors, such as eIF4E, is a hallmark of many cancers, making components of this machinery potential therapeutic targets [4]. However, because translation is a fundamental process required for the survival of all healthy cells, drugs that broadly inhibit the host translation machinery often exhibit a narrow therapeutic window and significant toxicity [5].

Other names

Protein synthesis machineryRibosome-mediated translationeGFP reporter systemHost cell translation apparatus

Inhibition of the eukaryotic ribosome or associated translation initiation and elongation factors to prevent the synthesis of new proteins.