MicroRNA 548b (miR-548b)

MicroRNA 548b is a small non-coding RNA molecule (20–24 nucleotides) categorized within the miR-548 family. It regulates gene expression at the post-transcriptional level by binding to complementary sequences in target mRNAs, resulting in their degradation or translational suppression. miR-548b is frequently downregulated in several malignancies, notably hepatocellular carcinoma, where its loss is associated with aggressive tumor features. Restoration of miR-548b inhibits tumor cell proliferation, colony formation, migration, invasion, and activates apoptosis. Mechanistically, miR-548b targets high-mobility group box 1 (HMGB1) and suppresses PI3K–AKT pathway activation. It is proposed as a candidate biomarker and therapeutic target for cancer diagnosis and treatment, especially in liver cancer and primary CNS lymphoma. There are no major issues with the target’s designation. It is a well-characterized microRNA, but not a receptor, enzyme, channel, or transporter; its therapeutic target status relates to RNA-based interventions rather than classical pharmacology.

Other names

MIR548Bhsa-mir-548bMIRN548BmiR-548b-5p

Therapeutic strategies may include miRNA mimics or inhibitors (antagomirs) to restore or block its function. Downregulates specific mRNA targets by binding to their 3′-UTR, causing transcript degradation or translation inhibition (e.g., HMGB1, MTA2).