NADH-dependent oxidoreductase

NADH-dependent oxidoreductases are a broad class of enzymes that catalyze the transfer of electrons from NADH to various electron acceptors, such as flavins (FMN, FAD), quinones, or ferredoxins, playing a central role in cellular redox metabolism and energy production[1][2][3][4][5]. These enzymes often contain flavin cofactors and share conserved structural motifs but can differ in substrate specificity and physiological roles depending on the organism and context[3][4]. Important representatives include NADH:FMN oxidoreductase, NADH:quinone oxidoreductase (NDH-2), and ferredoxin:NADP+ oxidoreductase (Nfn), among others[2][4]. Their physiological functions extend across oxidative phosphorylation in mitochondria, metabolic fermentation in bacteria, and redox homeostasis in various cell types. They are considered potential therapeutic targets in cancer and infection due to their involvement in mitochondrial and bacterial electron transport, but selectivity and safety remain significant challenges[4].

Other names

NADH:FMN oxidoreductaseNADH:quinone oxidoreductaseNADH oxidaseNADH dehydrogenaseFerredoxin:NADP+ oxidoreductase (for some enzyme classes)Flavoprotein oxidoreductase

Competitive or noncompetitive inhibition of electron transfer from NADH to acceptors (such as FMN, quinone, or ferredoxin)[1][2][4][5] Modulation of mitochondrial or bacterial electron transport chain