Nuclear genomic double-stranded DNA (None)

Nuclear genomic double-stranded DNA is the target of cyclophosphamide, an alkylating agent used in cancer chemotherapy and immunosuppression. Cyclophosphamide's active metabolite, phosphoramide mustard, alkylates DNA bases, predominantly guanine, causing DNA cross-linking, strand breaks, and base mispairing. These modifications disrupt DNA replication and transcription, leading to cell cycle arrest and apoptosis, particularly in rapidly dividing cells like cancer cells and activated immune cells.

Alkylation of DNA bases (primarily guanine) leading to DNA cross-linking, strand breaks, and inhibition of DNA replication and transcription, ultimately resulting in cell cycle arrest and apoptosis.