Nucleic acid sequence complementary to the probe

A "nucleic acid sequence complementary to the probe" refers to any single-stranded region of DNA or RNA that is able to hybridize, via standard base-pairing, to a synthetic nucleic acid probe specifically designed to match it[1][2][3][4][5][6][7]. This principle is fundamental to molecular diagnostic methods such as Southern blot, Northern blot, in situ hybridization, and quantitative PCR, in which the presence or abundance of a particular genetic sequence is assessed by the binding of a labeled probe[1][2][3][4][5][6][7][8]. The complementary sequence itself is not a distinct biological entity, protein, or receptor, and does not represent a therapeutic target, but rather a general concept in nucleic acid chemistry for sequence-specific detection.

Hybridization (binding of a probe to its complementary nucleic acid sequence for detection or quantification)[1][4][5][6][7][8]